Difference between revisions of "Introduction to Microarray analysis"
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====Overview of process==== | ====Overview of process==== | ||
[[Image:expt2.tiff|500px]] | [[Image:expt2.tiff|500px]] | ||
| + | *competitive hybridization to spotted oligo/cDNA transcripts | ||
| + | *Interested in genes that change between treatments | ||
| + | → ''differential expression'' | ||
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[[Image:overview2.tiff|thumb|Analysis workflow from scanner to results]] | [[Image:overview2.tiff|thumb|Analysis workflow from scanner to results]] | ||
Revision as of 01:44, 14 March 2006
Overview of process
- competitive hybridization to spotted oligo/cDNA transcripts
- Interested in genes that change between treatments
→ differential expression
Scratch pad
- A flow diagram for analysis
- Recap of cDNA microarrays (slide 3)
- Microarray data issues (slide 4)
- Microarray data issues (continued)
- Large amount of data (GPR/JPEG file size)
- Subjective
- Need a log of what was done so someone else can quickly reroduce the results
- → Reproducible research (someone else can understand/reproduce the results) (McGintys talk)
- Analysis process
- R resources/contributed guides (including downloading)
- R tutorial of basics (objects/indexing/functions)
