OR1Expt.R

Our Perl scripts. {{#security: Sven,Goldfinger}}

1. ------------------------- OR1 dataset analysis ------------------------------ #

if (.Platform$OS.type == 'unix' ) {

 sourceDir <- "/Volumes/HD2/Clinton/Data"

} else {

 sourceDir <- "C:/DATA/2007"

} filename <- dir(sourceDir, pattern="OR1.+\.txt")

1. Check file path

cat("Filename is:" ,sep="\n") cat(filename, sep="\n") cat("Full path is:", sep="\n") cat(file.path(sourceDir, filename), sep="\n")

dset <- read.table(file.path(sourceDir, filename), sep="\t", header=TRUE)

dim(dset) summary(dset)

1. ----------------------------- Dataset variables ----------------------------- #
2. Amount = 1,2,5 mu/g ingestion/injection
3. Timepoint = 1,2,3,4 days
4. Treatment OR1 gene/Sucrose control
5. AM = Administration method; feeding or injection
6. Response = RE (relative expression)

1. Took each treatment, prescribed an amount and feed/injected catapillars at
2. time 0. Adult moths were distructively assessed 1,2,3,4 days after emergence.
3. No catapillars were harmed during this experiment!
4. ~.~.~ Note: Sucrose control was only administered at Amount=1 ~.~.~

1. Convert names

colnames(dset)[4:5] <- c("AM","RE")

library(lattice)

1. Formulas
2. y ~ x
3. y ~ x | z
4. y ~ x | (z * w)

1. help(xyplot), help(panel.superpose)

xyplot( RE ~ AM, data=dset)#, key=mykey)

1. help(simpleKey)

mykey <- simpleKey(text=paste("Amount =",unique(dset$Amount)), space="top", columns=3) xyplot( RE ~ Timepoint| Treatment * AM, data=dset, panel.superpose=dset$Amount, groups=Amount, key=mykey)

1. An example

xyplot( RE ~ Treatment| Timepoint , data=dset, panel.superpose=dset$Amount, groups=Amount, key=mykey, layout=c(4,1), between=list(x=0.3), aspect=1)

1. Anova example
2. help(aov), help(summary)

aovobj <- aov(RE ~ AM*Treatment, data=dset) summary(aovobj)