Introduction to Microarray analysis
From Organic Design wiki
Overview of process
- competitive hybridization to spotted oligo/cDNA transcripts
- Interested in genes that change between treatments
- → differential expression
Scratch pad
- A flow diagram for analysis
- Recap of cDNA microarrays (slide 3)
- Microarray data issues (slide 4)
- Microarray data issues (continued)
- Large amount of data (GPR/JPEG file size)
- Subjective
- Need a log of what was done so someone else can quickly reroduce the results
- → Reproducible research (someone else can understand/reproduce the results) (McGintys talk)
- Analysis process
- R resources/contributed guides (including downloading)
- R tutorial of basics (objects/indexing/functions)
