Rosaceae

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Revision as of 21:02, 13 March 2006 by Sven (talk | contribs)

Microarray analysis workshop

Time schedule: 8:30 - 10:30am, 11-12:30am (3.5 hours)

Workflow

  1. Introduction to Microarray analysis (10mins - Marcus Davy)
  2. Normalization (10mins - Robert Schaffer)
  3. Bioconductor/R framework (15 mins - Marcus Davy)
  4. R tutorial (45mins)
  5. Linear models for Microarray analysis (limma) (15 mins - Marcus Davy)
  6. Experimental analysis ("45mins")

+Bioconductor/R framework

+R tasklist


Brief about Limma (10-15 mins)
  • Essentially t-statistics for each spot/gene
  • Uses between gene information in moderated t-statistics
  • Computationally fast/robust
  • Handles missing information/use defined flag information
  • benefits/limitations?
  • FDR control? → ranking better than selecting cutoff
Analysis script (1+hours)

+Workshop.R

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